By Harry ed. Sobotka
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Extra info for Advances in Clinical Chemistry, Vol. 4
The finding of a urine which has a positive Benedict reaction, but a negative glucose oxidase reaction, is the simplest screening method for the detection of nonglucose melliturias. 2. PRESERVATION Urine specimens which are collected for identification and quantitation of the sugar(s) present can be adequately preserved by adding toluene to the container (4-5 ml for a 24-hour collection) and keeping the urine cool until processed. It is important that the studies be started as soon as the collection is completed because urine becomes alkaline on standing and epimerization occurs ( P3).
This is quite well recognized and can be readily overcome. Together with variation in flame characteristics between test and standard reading, this is the only type of interference in which a lithium internal standard is really effective. 5. Self-absorption This refers to the failure of emission to increase in proportion to solution concentration above a certain limiting concentration. It is due to the absorption by atoms in the ground state in the cooler outer part of the flame of light emitted by excited atoms in the central hotter part of the flame.
The ash is dissolved in NHCl (200 ml HCl/g dry feces). The solution is thoroughly shaken to ensure complete dissolution. The solution is further diluted as required with diluting fluid (2). Soft Tissues. Soft tissues are dried to constant weight at 105" and then ground in an agate mortar with several changes of a mixture of equal 26 I. p. 40-60"). Obvious tendon is removed in the case of muscle. The powder is placed in the oven at 105"for 12 hours. A known weight (approximately 20 mg) of the dry fatfree tissue powder is weighed into a platinum crucible and ashed at 400".
Advances in Clinical Chemistry, Vol. 4 by Harry ed. Sobotka